Journal: Journal of Ovarian Research

Article Title: Expression pattern of circadian genes and steroidogenesis-related genes after testosterone stimulation in the human ovary

doi: 10.1186/s13048-016-0264-5

Figure Lengend Snippet: Immunohistochemistry staining of CLOCK and PER2 in paraffin sections of human ovaries. Staining of CLOCK was detected in the cumulus cells and mural granulosa cells, absent in the theca cells of the dominant antral follicles (D2, E2), and present in the interstitial cells, but absent in primordial follicles (A2), primary follicles (B2), and preantral follicles (C2). Staining of PER2 was present in the cumulus cells, mural granulosa cells, weak in the theca cells of dominant antral follicles (D3, E3), and present in the interstitial cells, but absent in the primordial follicles (A3), primary follicles (B3), and preantral follicles (C3). A1 to E1 are negative controls (no primary antibody) of the primordial, primary, preantral, and antral follicles and the cumulus complex, respectively. Bars = 50 μm. Original magnification, ×200

Article Snippet: Slides were washed and incubated with biotin-labeled goat anti-rabbit secondary antibody for CLOCK (CWBIO) or bovine anti-goat secondary antibody for PER2 (Santa Cruz Biotechnology) for 30 min, then washed and incubated with horseradish peroxidase-labeled streptavidin for 10 min.

Techniques: Immunohistochemistry, Staining

Journal: Journal of Ovarian Research

Article Title: Expression pattern of circadian genes and steroidogenesis-related genes after testosterone stimulation in the human ovary

doi: 10.1186/s13048-016-0264-5

Figure Lengend Snippet: Expression patterns of circadian genes after testosterone treatment in human luteinized granulosa cells. Human luteinized granulosa cells were exposed to 100 ng/mL testosterone dissolved in serum-free medium for 2 h and cells in the control group were cultured in serum-free medium without treatment. Samples were harvested every 4 h from the beginning of treatment for 48 h. Each value represents the mean ± SEM of three independent experiments. Significant statistical differences are shown as below: the testosterone group PER2 P 4 vs. 24 = 0.028, P 24 vs. 32 = 0.041, P 24 vs. 48 = 0.039, P 4 vs. 44 = 0.024, CLOCK P 4 vs. 24 = 0.04; the control group PER2 P 4 vs. 16 = 0.016, CLOCK P 4 vs. 12 = 0.022, P 4 vs. 16 = 0.031, P 4 vs. 20 = 0.006, P 4 vs. 36 = 0.011

Article Snippet: Slides were washed and incubated with biotin-labeled goat anti-rabbit secondary antibody for CLOCK (CWBIO) or bovine anti-goat secondary antibody for PER2 (Santa Cruz Biotechnology) for 30 min, then washed and incubated with horseradish peroxidase-labeled streptavidin for 10 min.

Techniques: Expressing, Cell Culture